Rabbits Colitis, Disease and Treatment: Suggestion for Research Study

arious immunosuppressive drugs have been mainly used for treatment of inflammatory bowel disease (IBD); however, their side effects and toxicity remain a major clinical concern. As a result, there is an increasing interest in using herbal medicine as an alternative and adjunct treatment in addition to the conventional therapies. As a suitable rabbit colitis model is currently not available, we developed a rabbit colitis model by administration of dextran sodium sulphate (DSS). Extraction of Dill Oil (DO) and Fennel Oil (FO). White Himalayan rabbits were acclimatized for 7days and then separated into five groups each of five rabbits. Group (1) (-ve) control, Group (2) DSS (+ve) control, Group(3) DSS+DO (1ml/day,5%), Group (4)DSS+FO (1ml/day,3.5%) and Group (5) DSS+DO+FO (0.5ml each per day). At the end of the experiment, rabbits will be sacrificed and the entire colon will be excised. Total glutathione will be determined in the colon homogente as well as, total RNA will be isolated from the colon homogenate, quantitative real-time PCR (qRT-PCR) reactions will be performed. Quantification of gene expression, Rabbit-specific primers will be designed for the genes of interest. The housekeeping gene βactin will be used as an internal control, and quantification of the transcripts will be performed by the {Delta}{Delta}CT method. Data will be expressed as mean± standard error of means (SEM). Comparisons will be performed by one-way ANOVA. Expected outcomes Upon detection of new treatments will be the entrance to produce a potent therapeutic preparation can be manufactured locally and marketed after making stages rely followed.